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What We Offer

Image by Trnava University
Image by CDC
Image by National Cancer Institute

Our Services

In vitro Bioassays

01

  • Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoal, Antialgal, Anticandidal)

  • Anticancer activity (Cytotoxicity, DNA fragmentation, Apoptotic Protein, Clonogenic assay)

  • Antioxidant activity (DPPH / ABTS scavenging, FRAP, TRAP, Reducing power, etc.)

  • Anti-inflammatory, Anti-hyperlipidemia

  • Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics,)

  • Wound healing study

  • Anti-coagulant activity

  • Anti-thrombotic activity

  • Membrane stabilization test

  • Heat-induced hemolysis

  • Cytoprotective assay

  • Endocrine disruptor screening

Microbial Assays

02

  • Antimicrobial Susceptibility Test  

  • Antibiotics Selection for Mastitis treatment (Clinical and Subclinical)

  • Veterinary Microbial Identification & Characterization

  • Bacterial identification

  • Fungal Identification

  • Total viable Bacterial count

  • Biochemical confirmatory tests of pathogens

  • Swab testing

  • Anti-viral, Anti-bacterial, and Anti-fungal assays

  • Isolation, culturing and media optimization of microbes

  • Metabolite screening and analysis

Molecular Techniques

03

  • DNA/ RNA/ protein Isolation & quantification

  • cDNA conversion

  • Flow cytometry

  • Agarose Gel Electrophoresis

  • PCR

  • ELISA

  • Western Blot

  • Real-Time PCR

Proteomics

04

  • Protein analysis

  • Protein purification and characterization

  • Extraction and purification of Enzymes

  • Protein extraction (Physical and chemical)

  • Protein precipitation (Ammonium sulphate precipitation)

  • Protein separation (SDS – PAGE)

  • Protein purification (Column Chromatography)

  • Protein estimation (Bradford Method)

  • Protein confirmation (Western blotting)

  • SDS-PAGE Gel Staining methods (Coomassie, Colloidal, Silver)

  • Estimation of bio molecules (Glucose, cholesterol etc)

CRO Services

1. Custom Peptide Antigen Synthesis

Our peptide design and synthesis services include modifications such as phosphorylation, acetylation, and biotinylation. A wide range of carrier proteins is available.

Customers provides

  • Amino acid sequence of the target protein

Service procedures

  • Peptide design

  • Peptide synthesis

  • Peptide conjugation

Deliverables

  • Peptide sequences

  • 2mg unconjugated peptide

2. ELISA Kit Development Service

Antigen preparation 

(Optional)

  • Rabbit pAb generation

Antibody generation 

ELISA Kit development

  • Antibody labeling

  • Antibody matched pair screening

  • Assay development and optimization

  • ELISA Kit manufacturing

Deliverables

  • Validated antibody pair set

  • CoA

3. Custom Rabbit Polyclonal Antibody Production Service

  • SDS-PAGE and UV analysis

Antigen validation

  • Pre-immune bleed

  • 2 rabbits immunization

  • Serum titer test

  • Final bleed

Standard immunization and

serum titer test

Purification
(Optional)

  • Protein A purification

  • Antigen affinity purification (As per customer's request)

  • SDS-PAGE and UV analysis

  • ELISA validation

QC analysis

Deliverables

  • Pre-immune sera, 2*0.5 mL/rabbit

  • Antisera or purified antibodies

  • CoA

  • Remaining antigens

4. E.coli Protein Expression Service

Gene synthesis and codon optimization (Optional)

  • Simply provide us with the protein/gene sequence or the plasmid.

Vector construction

  • Cloning cDNA into expression vectors

  • Plasmid sequencing

  • Plasmid preparation 

Deliverables

  • Sequencing report (if requested)

Protein expression testing

  • E. coli strain transformation

  • Pilot expression and purification trial

  • Protein expression evaluation (SDS-PAGE)

Deliverables

  • Expression evaluation result

  • Soluble protein purification

  • Protein refolding and purification (inclusion bodies)

  • QC analysis (SDS-PAGE, UV, etc)

Deliverables

  • 0.5-1 mg purified proteins (if feasible)

  • CoA

Scalable expression and 1-2 step purification

Large-scale expression and purification (Optional)

  • 100+ mg

  • QC analysis

Deliverables

  • Purified proteins

  • CoA

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